NOTCH4 Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61337r-cy3-100uL |
| Product Name | NOTCH4 Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
FLOW-CYT
|
| Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Notch 4 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4855 |
| Swiss Prot | Q99466 |
| Subcellular location | Nucleus, Cell membrane |
| Synonyms | NOTC4; hNotch4; INT3; Notch homolog 4. |
| Background | Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system. |
| Application Dilution | |
| WB | 1:300-5000 |
| Flow-Cyt | 1ug/Test |
Frequently asked questions
Which applications is NOTCH4 Recombinant Antibody, Cy3 Conjugated (bsm-61337R-Cy3) validated for, and at what dilution?
bsm-61337R-Cy3 is validated for WB (1:300-5000); Flow-Cyt (1ug/Test). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NOTCH4 Recombinant Antibody, Cy3 Conjugated (bsm-61337R-Cy3) react with?
Tested reactivity: Human, Mouse.
How should bsm-61337R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NOTCH4 Recombinant Antibody, Cy3 Conjugated (bsm-61337R-Cy3)?
KLH conjugated synthetic peptide derived from human Notch 4
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-61337R-Cy3)?
Cy3 on bsm-61337R-Cy3: excitation / emission 512,550nm/570,615nm.