HSP47 Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61347r-cy3-100uL |
| Product Name | HSP47 Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Serpin H1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 871 |
| Swiss Prot | P50454 |
| Subcellular location | Cytoplasm |
| Synonyms | CBP1; CBP2; OI10; gp46; AsTP3; HSP47; PIG14; PPROM; RA-A47; SERPINH2. |
| Background | This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum and plays a role in collagen biosynthesis as a collagen-specific molecular chaperone. Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
Frequently asked questions
Which applications is HSP47 Recombinant Antibody, Cy3 Conjugated (bsm-61347R-Cy3) validated for, and at what dilution?
bsm-61347R-Cy3 is validated for WB (1:300-5000); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HSP47 Recombinant Antibody, Cy3 Conjugated (bsm-61347R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61347R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HSP47 Recombinant Antibody, Cy3 Conjugated (bsm-61347R-Cy3)?
KLH conjugated synthetic peptide derived from human Serpin H1
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-61347R-Cy3)?
Cy3 on bsm-61347R-Cy3: excitation / emission 512,550nm/570,615nm.