CDC34 Recombinant Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61348r-20uL |
| Product Name | CDC34 Recombinant Antibody |
| Applications |
WB
IP
FLOW-CYT
ICC/IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CDC34 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 997 |
| Swiss Prot | P49427 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | CDC34; Cell division cycle 34; UBC3; UBCH3; Ubiquitin-protein ligase R1; UBE2R1; E2-CDC34; Ubiquitin carrier protein. |
| Background | Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation. |
| Application Dilution | |
| WB | 1:300-5000 |
| IP | 1-2ug |
| Flow-Cyt | 1ug/Test |
| ICC/IF | 1:50-100 |
Frequently asked questions
Which applications is CDC34 Recombinant Antibody (bsm-61348R) validated for, and at what dilution?
bsm-61348R is validated for WB (1:300-5000); IP (1-2ug); Flow-Cyt (1ug/Test); ICC/IF (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CDC34 Recombinant Antibody (bsm-61348R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61348R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
What immunogen was used to raise CDC34 Recombinant Antibody (bsm-61348R)?
KLH conjugated synthetic peptide derived from human CDC34