MGMT Recombinant Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61358r-20uL |
| Product Name | MGMT Recombinant Antibody |
| Applications |
WB
IHC-P
IHC-F
IF(IHC-P)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MGMT |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 4255 |
| Swiss Prot | P16455 |
| Subcellular location | Nucleus |
| Synonyms | MGMT |
| Background | Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. |
| Application Dilution | |
| WB | 1:500-2000 |
| IHC-P | IHC-P1:50-200 |
| IHC-F | IHC-F1:50-200 |
| IF(IHC-P) | IFIHC-P1:50-200 |
Frequently asked questions
Which applications is MGMT Recombinant Antibody (bsm-61358R) validated for, and at what dilution?
bsm-61358R is validated for WB(WB) (1:500-2000); IHC-P(IHC-P) (1:50-200); IHC-F(IHC-F) (1:50-200); IF(IHC-P)(IF(IHC-P)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MGMT Recombinant Antibody (bsm-61358R) react with?
Tested reactivity: Human.
How should bsm-61358R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
What immunogen was used to raise MGMT Recombinant Antibody (bsm-61358R)?
KLH conjugated synthetic peptide derived from human MGMT