SERCA2 Recombinant Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61408r-bf488-100uL |
| Product Name | SERCA2 Recombinant Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SERCA2 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 488 |
| Swiss Prot | P16615 |
| Subcellular location | Cytoplasm |
| Synonyms | SERCA2; Calcium pump 2; ATP2B. |
| Background | This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Involved in autophagy in response to starvation. Upon interaction with VMP1 and activation, controls ER-isolation membrane contacts for autophagosome formation. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is SERCA2 Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-61408R-BF488) validated for, and at what dilution?
bsm-61408R-BF488 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SERCA2 Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-61408R-BF488) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61408R-BF488 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SERCA2 Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-61408R-BF488)?
KLH conjugated synthetic peptide derived from human SERCA2
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bsm-61408R-BF488)?
AbBy Fluor-488 on bsm-61408R-BF488: excitation / emission 499nm/519nm.