ATG3 Recombinant Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61474r-100uL |
| Product Name | ATG3 Recombinant Antibody |
| Applications |
WB
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | A synthesized peptide derived from human ATG3 |
| Clonality | Recombinant |
| Clone # | 9B3 |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | purified by Protein A. |
| Storage Buffer | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 64422 |
| Swiss Prot | Q9NT62 |
| Subcellular location | Cytoplasm |
| Synonyms | APG3; APG3-LIKE; APG3L; PC3-96; hApg3; 2610016C12Rik; Atg3l; PIG-1; Pig1; ATG3_HUMAN; ATG3; Autophagy-related protein 3 (APG3-like | hApg3); Protein PC3-96; 2.3.2.-; ATG3_MOUSE; Autophagy-related protein 3 (APG3-like); ATG3_RAT; Preconditioning-inducible gene 1 protein; autophagy related 3; APG3 autophagy 3-like (S. cerevisiae); ATG3 autophagy related 3 homolog (S. cerevisiae) |
| Background | E2 conjugating enzyme that catalyzes the covalent conjugation of the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to the amino group of phosphatidylethanolamine (PE)-containing lipids in the membrane resulting in membrane-bound ATG8-like proteins which is one of the key steps in the development of autophagic isolation membranes during autophagosome formation. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is ATG3 Recombinant Antibody (bsm-61474R) validated for, and at what dilution?
bsm-61474R is validated for WB(WB) (1:500-2000); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ATG3 Recombinant Antibody (bsm-61474R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61474R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
What immunogen was used to raise ATG3 Recombinant Antibody (bsm-61474R)?
A synthesized peptide derived from human ATG3