WNT5A Recombinant Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61491r-100uL |
| Product Name | WNT5A Recombinant Antibody |
| Applications |
WB
FLOW-CYT
ICC/IF
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Wnt5a |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 7474 |
| Swiss Prot | P41221 |
| Subcellular location | Extracellular, Secreted |
| Synonyms | hWNT5A; Protein Wnt5a; Wingless type MMTV integration site family member 5A; WNT 5A protein; WNT 5A protein precursor; WNT5A. |
| Background | Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. |
| Application Dilution | |
| WB | 1:300-5000 |
| Flow-Cyt | FCM1:20-100 |
| ICC/IF | IFICC1:50-200 |
Frequently asked questions
Which applications is WNT5A Recombinant Antibody (bsm-61491R) validated for, and at what dilution?
bsm-61491R is validated for WB (1:300-5000); FCM (1:20-100); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does WNT5A Recombinant Antibody (bsm-61491R) react with?
Tested reactivity: Human.
How should bsm-61491R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
What immunogen was used to raise WNT5A Recombinant Antibody (bsm-61491R)?
KLH conjugated synthetic peptide derived from human Wnt5a