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GOLGA1 Recombinant Antibody

The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-GOLGA1 antibody (bsm-61533R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-GOLGA1 antibody (bsm-61533R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
25 ug total protein per lane of various lysates (see on figure) probed with GOLGA1 monoclonal antibody, unconjugated (bsm-61533R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with GOLGA1 monoclonal antibody, unconjugated (bsm-61533R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Bioss unconjugated antibody vial

Applications

  • FCM

Reactivity

  • Human
Overview
Catalog # bsm-61533r-100uL
Product Name GOLGA1 Recombinant Antibody
Applications
FCM
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Golgin subfamily A member 1
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 2800
Swiss Prot Q92805
Subcellular location Cytoplasm, Cell membrane
Synonyms Gap junction protein alpha 4 37kD; GOLGA1; Golgi autoantigen golgin subfamily a 1; Golgin A1; Golgin subfamily A member 1; Golgin97.
Background Involved in vesicular trafficking at the Golgi apparatus level. Involved in endosome-to-Golgi trafficking.
Application Dilution
FCM 1:50-100

Frequently asked questions

Which applications is GOLGA1 Recombinant Antibody (bsm-61533R) validated for, and at what dilution?

bsm-61533R is validated for FCM(FCM) (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does GOLGA1 Recombinant Antibody (bsm-61533R) react with?

Tested reactivity: Human.

How should bsm-61533R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

What immunogen was used to raise GOLGA1 Recombinant Antibody (bsm-61533R)?

KLH conjugated synthetic peptide derived from human Golgin subfamily A member 1

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