CYP3A4 Recombinant Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61566r-100uL |
| Product Name | CYP3A4 Recombinant Antibody |
| Applications |
WB
IHC-P
IHC-F
IF(IHC-P)
IP
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Cytochrome P450 3A4 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 1576 |
| Swiss Prot | P08684 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | CYP3A4; CYPIIIA3; CYPIIIA4; Cytochrome P450 3A3; Cytochrome P450 HLp; Cytochrome P450 NF-25; Cytochrome P450-PCN1; Nifedipine oxidase. |
| Background | A cytochrome P450 monooxygenase involved in the metabolism of sterols, steroid hormones, retinoids and fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. |
| Application Dilution | |
| WB | 1:500-2000 |
| IHC-P | IHC-P1:100-500 |
| IHC-F | IHC-F1:100-500 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IP | IP1:20-50 |
Frequently asked questions
Which applications is CYP3A4 Recombinant Antibody (bsm-61566R) validated for, and at what dilution?
bsm-61566R is validated for WB(WB) (1:500-2000); IHC-P(IHC-P) (1:100-500); IHC-F(IHC-F) (1:100-500); IF(IHC-P)(IF(IHC-P)) (1:100-500); IP(IP) (1:20-50). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CYP3A4 Recombinant Antibody (bsm-61566R) react with?
Tested reactivity: Human.
How should bsm-61566R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
What immunogen was used to raise CYP3A4 Recombinant Antibody (bsm-61566R)?
KLH conjugated synthetic peptide derived from human Cytochrome P450 3A4