PHD2 Recombinant Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61581r-100uL |
| Product Name | PHD2 Recombinant Antibody |
| Applications |
WB
IP
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PHD2 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 54583 |
| Swiss Prot | Q9GZT9 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | EGLN1; C1orf12; ECYT3; HIFPH2; HPH2; PHD2; SM-20; SM20; ZMYND6. |
| Background | Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF1B. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. |
| Application Dilution | |
| WB | 1:300-5000 |
| IP | 1-2ug |
Frequently asked questions
Which applications is PHD2 Recombinant Antibody (bsm-61581R) validated for, and at what dilution?
bsm-61581R is validated for WB (1:300-5000); IP (1-2ug). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PHD2 Recombinant Antibody (bsm-61581R) react with?
Tested reactivity: Human.
How should bsm-61581R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
What immunogen was used to raise PHD2 Recombinant Antibody (bsm-61581R)?
KLH conjugated synthetic peptide derived from human PHD2