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ARA24 Recombinant Antibody

4% Paraformaldehyde-fixed Jurkat (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (ARA24) monoclonal Antibody, unconjugated (bsm-61597R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed Jurkat (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (ARA24) monoclonal Antibody, unconjugated (bsm-61597R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The Jurkat (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-ARA24 antibody (bsm-61597R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The Jurkat (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-ARA24 antibody (bsm-61597R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Bioss unconjugated antibody vial

Applications

  • WB
  • IP
  • Flow-Cyt
  • ICC/IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-61597r-100uL
Product Name ARA24 Recombinant Antibody
Applications
WB IP FLOW-CYT ICC/IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human RAN
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 5901
Swiss Prot P62826
Subcellular location Cytoplasm, Nucleus
Synonyms ARA24; Gsp1; GTPase Ran; Guanosine triphosphatase Ran; LPS; RAN member RAS oncogene family; RanGTPase; Ras like protein TC4; Ras related nuclear protein; Ras-like protein TC4; RASL2 8.
Background GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle .
Application Dilution
WB 1:300-5000
IP FCM1:20-100
Flow-Cyt IFICC1:50-200
ICC/IF IP1-2ug

Frequently asked questions

Which applications is ARA24 Recombinant Antibody (bsm-61597R) validated for, and at what dilution?

bsm-61597R is validated for WB (1:300-5000); FCM (1:20-100); IF(ICC) (1:50-200); IP (1-2ug). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ARA24 Recombinant Antibody (bsm-61597R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-61597R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

What immunogen was used to raise ARA24 Recombinant Antibody (bsm-61597R)?

KLH conjugated synthetic peptide derived from human RAN

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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