Calmegin Recombinant Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61616r-rbitc-100uL |
| Product Name | Calmegin Recombinant Antibody, RBITC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Calmegin |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1047 |
| Swiss Prot | O14967 |
| Synonyms | CLGN; MEG 1 antigen; Calnexin-T; Calmegin precursor; Calmegin. |
| Background | Functions during spermatogenesis as a chaperone for a range of client proteins that are important for sperm adhesion onto the egg zona pellucida and for subsequent penetration of the zona pellucida. Required for normal sperm migration from the uterus into the oviduct. Required for normal male fertility. Binds calcium ions. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is Calmegin Recombinant Antibody, RBITC Conjugated (bsm-61616R-RBITC) validated for, and at what dilution?
bsm-61616R-RBITC is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Calmegin Recombinant Antibody, RBITC Conjugated (bsm-61616R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61616R-RBITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Calmegin Recombinant Antibody, RBITC Conjugated (bsm-61616R-RBITC)?
KLH conjugated synthetic peptide derived from human Calmegin
What are the excitation and emission wavelengths of the RBITC conjugate (bsm-61616R-RBITC)?
RBITC on bsm-61616R-RBITC: excitation / emission 570nm/595nm.