MELK Recombinant Antibody, PE Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61708r-pe-100uL |
| Product Name | MELK Recombinant Antibody, PE Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MELK |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9833 |
| Swiss Prot | Q14680 |
| Subcellular location | Cytoplasm |
| Synonyms | hMELK; hPK38; MELK; mKIAA0175; MPK38. |
| Background | Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. |
| Application Dilution | |
| WB | 1:500-2000 |
Frequently asked questions
Which applications is MELK Recombinant Antibody, PE Conjugated (bsm-61708R-PE) validated for, and at what dilution?
bsm-61708R-PE is validated for WB(WB) (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MELK Recombinant Antibody, PE Conjugated (bsm-61708R-PE) react with?
Tested reactivity: Human.
How should bsm-61708R-PE be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MELK Recombinant Antibody, PE Conjugated (bsm-61708R-PE)?
KLH conjugated synthetic peptide derived from human MELK
What are the excitation and emission wavelengths of the PE conjugate (bsm-61708R-PE)?
PE on bsm-61708R-PE: excitation / emission 496,564nm/578nm.