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Prostate Secretory Protein/PSP Recombinant Antibody

25 ug total protein per lane of various lysates (see on figure) probed with Prostate Secretory Protein\/PSP monoclonal antibody, unconjugated (bsm-61761R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with Prostate Secretory Protein\/PSP monoclonal antibody, unconjugated (bsm-61761R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Prostate Secretory Protein\/PSP Monoclonal Antibody, Unconjugated (bsm-61761R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Prostate Secretory Protein\/PSP Monoclonal Antibody, Unconjugated (bsm-61761R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Prostate Secretory Protein\/PSP Monoclonal Antibody, Unconjugated (bsm-61761R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Prostate Secretory Protein\/PSP Monoclonal Antibody, Unconjugated (bsm-61761R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver\r\n; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Prostate Secretory Protein\/PSP Monoclonal Antibody, Unconjugated (bsm-61761R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver\r\n; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Prostate Secretory Protein\/PSP Monoclonal Antibody, Unconjugated (bsm-61761R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Prostate Secretory Protein\/PSP Monoclonal Antibody, Unconjugated (bsm-61761R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Prostate Secretory Protein\/PSP Monoclonal Antibody, Unconjugated (bsm-61761R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IP

Reactivity

  • Human
Overview
Catalog # bsm-61761r-20uL
Product Name Prostate Secretory Protein/PSP Recombinant Antibody
Applications
WB IHC-P IHC-F IF(IHC-P) IP
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human beta 2 Microseminoprotein
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 4477
Swiss Prot P08118
Subcellular location Secreted
Synonyms MSP; PSP; IGBF; MSPB; PN44; PRPS; HPC13; PSP57; PSP94; PSP-94.
Background The protein encoded by this gene is a member of the immunoglobulin binding factor family. It is synthesized by the epithelial cells of the prostate gland and secreted into the seminal plasma. This protein has inhibin-like activity. It may have a role as an autocrine paracrine factor in uterine, breast and other female reproductive tissues. The expression of the encoded protein is found to be decreased in prostate cancer.
Application Dilution
WB 1:500-2000
IHC-P IHC-P1:200-1000
IHC-F IHC-F1:200-1000
IF(IHC-P) IFIHC-P1:200-1000
IP IP1:20-50

Frequently asked questions

Which applications is Prostate Secretory Protein/PSP Recombinant Antibody (bsm-61761R) validated for, and at what dilution?

bsm-61761R is validated for WB(WB) (1:500-2000); IHC-P(IHC-P) (1:200-1000); IHC-F(IHC-F) (1:200-1000); IF(IHC-P)(IF(IHC-P)) (1:200-1000); IP(IP) (1:20-50). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Prostate Secretory Protein/PSP Recombinant Antibody (bsm-61761R) react with?

Tested reactivity: Human.

How should bsm-61761R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

What immunogen was used to raise Prostate Secretory Protein/PSP Recombinant Antibody (bsm-61761R)?

KLH conjugated synthetic peptide derived from human beta 2 Microseminoprotein

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