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ABCD3 Recombinant Antibody

4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (ABCD3) monoclonal Antibody, unconjugated (bsm-61771R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (ABCD3) monoclonal Antibody, unconjugated (bsm-61771R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-ABCD3 antibody (bsm-61771R\uff0c1:100\uff09; Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (bs-60295G-BF488): 1 \u03bcg\/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-ABCD3 antibody (bsm-61771R\uff0c1:100\uff09; Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (bs-60295G-BF488): 1 \u03bcg\/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (ABCD3) monoclonal Antibody, unconjugated (bsm-61771R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, then stained with Phalloidin\/BF555 for 30 min at room temperature; DAPI (blue, C02-04002) was used to stain the cell nuclei.
4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (ABCD3) monoclonal Antibody, unconjugated (bsm-61771R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, then stained with Phalloidin\/BF555 for 30 min at room temperature; DAPI (blue, C02-04002) was used to stain the cell nuclei.
4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (ABCD3) monoclonal Antibody, unconjugated (bsm-61771R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min; DAPI (blue, C02-04002) was used to stain the cell nuclei.
4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (ABCD3) monoclonal Antibody, unconjugated (bsm-61771R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Bioss unconjugated antibody vial

Applications

  • FCM
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-61771r-20uL
Product Name ABCD3 Recombinant Antibody
Applications
FCM IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ABCD3
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 5825
Swiss Prot P28288
Subcellular location Cytoplasm, Cell membrane
Synonyms ABCD3; ABC43; PMP70; PXMP1; ZWS2.
Background Probable transporter. The nucleotide-binding fold acts as an ATP-binding subunit with ATPase activity.
Application Dilution
FCM 1:50-100
IF(ICC) IFICC1:50-200

Frequently asked questions

Which applications is ABCD3 Recombinant Antibody (bsm-61771R) validated for, and at what dilution?

bsm-61771R is validated for FCM(FCM) (1:50-100); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ABCD3 Recombinant Antibody (bsm-61771R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-61771R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

What immunogen was used to raise ABCD3 Recombinant Antibody (bsm-61771R)?

KLH conjugated synthetic peptide derived from human ABCD3

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