Bub1 Recombinant Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61781r-100uL |
| Product Name | Bub1 Recombinant Antibody |
| Applications |
WB
IHC-P
IHC-F
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human BUB1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 699 |
| Swiss Prot | O43683 |
| Subcellular location | Nucleus |
| Synonyms | Bub1; BUB1A; BUB1L; hBUB1. |
| Background | Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF | |
Frequently asked questions
Which applications is Bub1 Recombinant Antibody (bsm-61781R) validated for, and at what dilution?
bsm-61781R is validated for WB (1:300-5000); IHC-P (1:200-400); IHC-F (1:100-500); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Bub1 Recombinant Antibody (bsm-61781R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61781R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
What immunogen was used to raise Bub1 Recombinant Antibody (bsm-61781R)?
KLH conjugated synthetic peptide derived from human BUB1