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PFDN5 Recombinant Antibody

25 ug total protein per lane of various lysates (see on figure) probed with PFDN5 monoclonal antibody, unconjugated (bsm-61863R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with PFDN5 monoclonal antibody, unconjugated (bsm-61863R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PFDN5 Monoclonal Antibody, Unconjugated (bsm-61863R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PFDN5 Monoclonal Antibody, Unconjugated (bsm-61863R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PFDN5 Monoclonal Antibody, Unconjugated (bsm-61863R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PFDN5 Monoclonal Antibody, Unconjugated (bsm-61863R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PFDN5 Monoclonal Antibody, Unconjugated (bsm-61863R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PFDN5 Monoclonal Antibody, Unconjugated (bsm-61863R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PFDN5 Monoclonal Antibody, Unconjugated (bsm-61863R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PFDN5 Monoclonal Antibody, Unconjugated (bsm-61863R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IP

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-61863r-100uL
Product Name PFDN5 Recombinant Antibody
Applications
WB IHC-P IHC-F IF(IHC-P) IP
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human PFDN5
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 5204
Swiss Prot Q99471
Subcellular location Cytoplasm, Nucleus
Synonyms EIG 1; Eig1; MM1; PFD5; PFDN5; Prefoldin 5.
Background Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. Represses the transcriptional activity of MYC.
Application Dilution
WB 1:500-2000
IHC-P IHC-P1:100-200
IHC-F IHC-F1:100-200
IF(IHC-P) IFIHC-P1:100-200
IP IP1:20-50

Frequently asked questions

Which applications is PFDN5 Recombinant Antibody (bsm-61863R) validated for, and at what dilution?

bsm-61863R is validated for WB(WB) (1:500-2000); IHC-P(IHC-P) (1:100-200); IHC-F(IHC-F) (1:100-200); IF(IHC-P)(IF(IHC-P)) (1:100-200); IP(IP) (1:20-50). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does PFDN5 Recombinant Antibody (bsm-61863R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-61863R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

What immunogen was used to raise PFDN5 Recombinant Antibody (bsm-61863R)?

KLH conjugated synthetic peptide derived from human PFDN5

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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