GRASP65 Recombinant Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61876r-cy5-100uL |
| Product Name | GRASP65 Recombinant Antibody, Cy5 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GORASP1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 64689 |
| Swiss Prot | Q9BQQ3 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | GOLPH5; GORASP1; GRASP65. |
| Background | Key structural protein of the Golgi apparatus.The membrane cisternae of the Golgi apparatus adhere to each other to form stacks, which are aligned side by side to form the Golgi ribbon.Acting in concert with GORASP2/GRASP55, is required for the formation and maintenance of the Golgi ribbon, and may be dispensable for the formation of stacks. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1μg/Test |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is GRASP65 Recombinant Antibody, Cy5 Conjugated (bsm-61876R-Cy5) validated for, and at what dilution?
bsm-61876R-Cy5 is validated for WB(WB) (1:500-2000); FCM(FCM) (1μg/Test); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GRASP65 Recombinant Antibody, Cy5 Conjugated (bsm-61876R-Cy5) react with?
Tested reactivity: Human.
How should bsm-61876R-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GRASP65 Recombinant Antibody, Cy5 Conjugated (bsm-61876R-Cy5)?
KLH conjugated synthetic peptide derived from human GORASP1
What are the excitation and emission wavelengths of the Cy5 conjugate (bsm-61876R-Cy5)?
Cy5 on bsm-61876R-Cy5: excitation / emission 625,650nm/670nm.