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PIST Recombinant Antibody

25 ug total protein per lane of various lysates (see on figure) probed with PIST monoclonal antibody, unconjugated (bsm-61917R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with PIST monoclonal antibody, unconjugated (bsm-61917R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The A375 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-PIST antibody (bsm-61917R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.\r\n
The A375 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-PIST antibody (bsm-61917R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.\r\n
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PIST Monoclonal Antibody, Unconjugated (bsm-61917R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PIST Monoclonal Antibody, Unconjugated (bsm-61917R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PIST Monoclonal Antibody, Unconjugated (bsm-61917R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PIST Monoclonal Antibody, Unconjugated (bsm-61917R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PIST Monoclonal Antibody, Unconjugated (bsm-61917R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PIST Monoclonal Antibody, Unconjugated (bsm-61917R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PIST Monoclonal Antibody, Unconjugated (bsm-61917R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PIST Monoclonal Antibody, Unconjugated (bsm-61917R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PIST Monoclonal Antibody, Unconjugated (bsm-61917R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PIST Monoclonal Antibody, Unconjugated (bsm-61917R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • FCM
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(ICC)
  • IP

Reactivity

  • Human
  • Mouse
Overview
Catalog # bsm-61917r-100uL
Product Name PIST Recombinant Antibody
Applications
WB FCM IHC-P IHC-F IF(IHC-P) IF(ICC) IP
Reactivity Human, Mouse
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human GOPC
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 57120
Swiss Prot Q9HD26
Subcellular location Cytoplasm, Cell membrane
Synonyms GOPC; CAL; CFTR-associated ligand; GOPC1; FIG; PIST.
Background Plays a role in intracellular protein trafficking and degradation.May regulate CFTR chloride currents and acid-induced ASIC3 currents by modulating cell surface expression of both channels.May also regulate the intracellular trafficking of the ADR1B receptor.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100
IHC-P IHC-P1:100-200
IHC-F IHC-F1:100-200
IF(IHC-P) IFIHC-P1:50-200
IF(ICC) IFICC1:50-200
IP IP1:20-50

Frequently asked questions

Which applications is PIST Recombinant Antibody (bsm-61917R) validated for, and at what dilution?

bsm-61917R is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IHC-P(IHC-P) (1:100-200); IHC-F(IHC-F) (1:100-200); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200); IP(IP) (1:20-50). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does PIST Recombinant Antibody (bsm-61917R) react with?

Tested reactivity: Human, Mouse.

How should bsm-61917R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

What immunogen was used to raise PIST Recombinant Antibody (bsm-61917R)?

KLH conjugated synthetic peptide derived from human GOPC

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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