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HIPK2 Recombinant Antibody

4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (HIPK2) monoclonal Antibody, unconjugated (bsm-62009R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (HIPK2) monoclonal Antibody, unconjugated (bsm-62009R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The MCF-7 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-HIPK2 antibody (bsm-62009R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The MCF-7 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-HIPK2 antibody (bsm-62009R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Bioss unconjugated antibody vial

Applications

  • WB
  • FCM
  • IF(ICC)
  • IP

Reactivity

  • Human
  • Mouse
Overview
Catalog # bsm-62009r-100uL
Product Name HIPK2 Recombinant Antibody
Applications
WB FCM IF(ICC) IP
Reactivity Human, Mouse
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human HIPK2
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 28996
Swiss Prot Q9H2X6
Subcellular location Cytoplasm, Nucleus
Synonyms hHIPk2; Hipk2; Nbak1; Stank.
Background Serine/threonine-protein kinase involved in transcription regulation, p53/TP53-mediated cellular apoptosis and regulation of the cell cycle. Acts as a corepressor of several transcription factors, including SMAD1 and POU4F1/Brn3a and probably NK homeodomain transcription factors. Phosphorylates PDX1, ATF1, PML, p53/TP53, CREB1, CTBP1, CBX4, RUNX1, EP300, CTNNB1, HMGA1, ZBTB4 and DAZAP2.
Application Dilution
WB 1:1000-2000
FCM FCM1μg/Test
IF(ICC) IFICC1:50-200
IP IP1:20-50

Frequently asked questions

Which applications is HIPK2 Recombinant Antibody (bsm-62009R) validated for, and at what dilution?

bsm-62009R is validated for WB(WB) (1:1000-2000); FCM(FCM) (1μg/Test); IF(ICC)(IF(ICC)) (1:50-200); IP(IP) (1:20-50). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does HIPK2 Recombinant Antibody (bsm-62009R) react with?

Tested reactivity: Human, Mouse.

How should bsm-62009R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

What immunogen was used to raise HIPK2 Recombinant Antibody (bsm-62009R)?

KLH conjugated synthetic peptide derived from human HIPK2

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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