POLD1 Recombinant Antibody, PE Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62042r-pe-100uL |
| Product Name | POLD1 Recombinant Antibody, PE Conjugated |
| Applications |
WB
IF
FLOW-CYT
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human POLD1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5424 |
| Swiss Prot | P28340 |
| Subcellular location | Nucleus |
| Synonyms | CDC2; CRCS10; MDPL; POLD; POLD1. |
| Background | Possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Required with its accessory proteins (proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) or activator 1) for leading strand synthesis. Also involved in completing Okazaki fragments initiated by the DNA polymerase alpha/primase complex. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
| Flow-Cyt | 1ug/Test |
Frequently asked questions
Which applications is POLD1 Recombinant Antibody, PE Conjugated (bsm-62042R-PE) validated for, and at what dilution?
bsm-62042R-PE is validated for WB (1:300-5000); IF; Flow-Cyt (1ug/Test). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does POLD1 Recombinant Antibody, PE Conjugated (bsm-62042R-PE) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62042R-PE be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise POLD1 Recombinant Antibody, PE Conjugated (bsm-62042R-PE)?
KLH conjugated synthetic peptide derived from human POLD1
What are the excitation and emission wavelengths of the PE conjugate (bsm-62042R-PE)?
PE on bsm-62042R-PE: excitation / emission 496,564nm/578nm.