NFIB Recombinant Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62113r-100uL |
| Product Name | NFIB Recombinant Antibody |
| Applications |
WB
IHC-P
IHC-F
IP
IF
FLOW-CYT
ICC/IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NFIB type |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 4781 |
| Swiss Prot | O00712 |
| Subcellular location | Nucleus |
| Synonyms | CTF; HMGIC/NFIB; NF-I/B; NF1-B; NF1B; NF1B2; NFI-B; NFI-RED; Nfib; NFIB2; NFIB3. |
| Background | Transcriptional activator of GFAP, essential for proper brain development.Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | FCM1:20-100 |
| IHC-F | IHC-P1:200-400 |
| IP | IHC-F1:100-500 |
| IF | ICC1:50-200 |
| Flow-Cyt | IP1-2ug |
| ICC/IF | IF |
Frequently asked questions
Which applications is NFIB Recombinant Antibody (bsm-62113R) validated for, and at what dilution?
bsm-62113R is validated for WB (1:300-5000); FCM (1:20-100); IHC-P (1:200-400); IHC-F (1:100-500); IF(ICC) (1:50-200); IP (1-2ug); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NFIB Recombinant Antibody (bsm-62113R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62113R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
What immunogen was used to raise NFIB Recombinant Antibody (bsm-62113R)?
KLH conjugated synthetic peptide derived from human NFIB type