PA26 Recombinant Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62280r-100uL |
| Product Name | PA26 Recombinant Antibody |
| Applications |
WB
FLOW-CYT
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Sestrin 1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 27244 |
| Swiss Prot | Q9Y6P5 |
| Subcellular location | Nucleus |
| Synonyms | PA26; sesn1; SEST1; sestrin 1. |
| Background | Functions as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway through the GATOR complex. In absence of leucine, binds the GATOR subcomplex GATOR2 and prevents TORC1 signaling. Binding of leucine to SESN2 disrupts its interaction with GATOR2 thereby activating the TORC1 signaling pathway. |
| Application Dilution | |
| WB | 1:300-5000 |
| Flow-Cyt | FCM1:20-100 |
Frequently asked questions
Which applications is PA26 Recombinant Antibody (bsm-62280R) validated for, and at what dilution?
bsm-62280R is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PA26 Recombinant Antibody (bsm-62280R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62280R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
What immunogen was used to raise PA26 Recombinant Antibody (bsm-62280R)?
KLH conjugated synthetic peptide derived from human Sestrin 1