SMG1 Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62379r-cy3-100uL |
| Product Name | SMG1 Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SMG1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 23049 |
| Swiss Prot | Q96Q15 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | 61E3.4; ATX; hSMG1; LIP; smg1. |
| Background | Serine/threonine protein kinase involved in both mRNA surveillance and genotoxic stress response pathways. Recognizes the substrate consensus sequence [ST]-Q. Plays a central role in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by phosphorylating UPF1/RENT1. Recruited by release factors to stalled ribosomes together with SMG8 and SMG9 (forming the SMG1C protein kinase complex), and UPF1 to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is SMG1 Recombinant Antibody, Cy3 Conjugated (bsm-62379R-Cy3) validated for, and at what dilution?
bsm-62379R-Cy3 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SMG1 Recombinant Antibody, Cy3 Conjugated (bsm-62379R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62379R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SMG1 Recombinant Antibody, Cy3 Conjugated (bsm-62379R-Cy3)?
KLH conjugated synthetic peptide derived from human SMG1
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-62379R-Cy3)?
Cy3 on bsm-62379R-Cy3: excitation / emission 512,550nm/570,615nm.