THEMIS Recombinant Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62442r-100uL |
| Product Name | THEMIS Recombinant Antibody |
| Applications |
WB
FCM
IF(ICC)
IP
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human THEMIS |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 387357 |
| Swiss Prot | Q8N1K5 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Protein THEMIS; SPOT; THEMIS; TSEPA. |
| Background | Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(ICC) | IFICC1:50-200 |
| IP | IP1:20-50 |
Frequently asked questions
Which applications is THEMIS Recombinant Antibody (bsm-62442R) validated for, and at what dilution?
bsm-62442R is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(ICC)(IF(ICC)) (1:50-200); IP(IP) (1:20-50). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does THEMIS Recombinant Antibody (bsm-62442R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-62442R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
What immunogen was used to raise THEMIS Recombinant Antibody (bsm-62442R)?
KLH conjugated synthetic peptide derived from human THEMIS