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PRPF31 Recombinant Antibody

25 ug total protein per lane of various lysates (see on figure) probed with PRPF31 monoclonal antibody, unconjugated (bsm-62711R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with PRPF31 monoclonal antibody, unconjugated (bsm-62711R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-PRPF31 antibody (bsm-62711R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.\r\n
The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-PRPF31 antibody (bsm-62711R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.\r\n
Bioss unconjugated antibody vial

Applications

  • WB
  • Flow-Cyt
  • ICC/IF

Reactivity

  • Human
Overview
Catalog # bsm-62711r-100uL
Product Name PRPF31 Recombinant Antibody
Applications
WB FLOW-CYT ICC/IF
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human PRPF31
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 26121
Swiss Prot Q8WWY3
Synonyms hPrp31; Protein 61K; PRP31; prpf31; RP11; SNRNP61.
Background Involved in pre-mRNA splicing as component of the spliceosome.Required for the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome.
Application Dilution
WB 1:300-5000
Flow-Cyt FCM1:20-100
ICC/IF IFICC1:50-200

Frequently asked questions

Which applications is PRPF31 Recombinant Antibody (bsm-62711R) validated for, and at what dilution?

bsm-62711R is validated for WB (1:300-5000); FCM (1:20-100); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does PRPF31 Recombinant Antibody (bsm-62711R) react with?

Tested reactivity: Human.

How should bsm-62711R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

What immunogen was used to raise PRPF31 Recombinant Antibody (bsm-62711R)?

KLH conjugated synthetic peptide derived from human PRPF31

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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