NOLC1 Recombinant Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62739r-bf488-100uL |
| Product Name | NOLC1 Recombinant Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NOLC1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9221 |
| Swiss Prot | Q14978 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | NOLC1; NOPP130; Nopp140; NS5ATP13; Nucleolar phosphoprotein p130; P130. |
| Background | Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification.Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with TCOF1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF | 1:50-200 |
Frequently asked questions
Which applications is NOLC1 Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-62739R-BF488) validated for, and at what dilution?
bsm-62739R-BF488 is validated for WB (1:500-2000); IF (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NOLC1 Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-62739R-BF488) react with?
Tested reactivity: Human.
How should bsm-62739R-BF488 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NOLC1 Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-62739R-BF488)?
KLH conjugated synthetic peptide derived from human NOLC1
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bsm-62739R-BF488)?
AbBy Fluor-488 on bsm-62739R-BF488: excitation / emission 499nm/519nm.