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TMEM67/Meckelin Recombinant Antibody

25 ug total protein per lane of various lysates (see on figure) probed with TMEM67\/Meckelin monoclonal antibody, unconjugated (bsm-62769R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with TMEM67\/Meckelin monoclonal antibody, unconjugated (bsm-62769R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed HepG2 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (TMEM67\/Meckelin) monoclonal Antibody, unconjugated (bsm-62769R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed HepG2 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (TMEM67\/Meckelin) monoclonal Antibody, unconjugated (bsm-62769R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The HepG2 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-TMEM67\/Meckelin antibody (bsm-62769R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The HepG2 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-TMEM67\/Meckelin antibody (bsm-62769R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF
  • Flow-Cyt
  • ICC/IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-62769r-100uL
Product Name TMEM67/Meckelin Recombinant Antibody
Applications
WB IHC-P IHC-F IF FLOW-CYT ICC/IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Meckelin
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 91147
Swiss Prot Q5HYA8
Subcellular location Cell membrane
Synonyms JBTS6; MKS3; NPHP11; TMEM67; TNEM67.
Background Required for ciliary structure and function. Part of the tectonic-like complex which is required for tissue-specific ciliogenesis and may regulate ciliary membrane composition.Involved in centrosome migration to the apical cell surface during early ciliogenesis. Involved in the regulation of cilia length and appropriate number through the control of centrosome duplication. Is a key regulator of stereociliary bundle orientation.
Application Dilution
WB 1:300-5000
IHC-P FCM1:20-100
IHC-F IHC-P1:200-400
IF IHC-F1:100-500
Flow-Cyt IFICC1:50-200
ICC/IF IF

Frequently asked questions

Which applications is TMEM67/Meckelin Recombinant Antibody (bsm-62769R) validated for, and at what dilution?

bsm-62769R is validated for WB (1:300-5000); FCM (1:20-100); IHC-P (1:200-400); IHC-F (1:100-500); IF(ICC) (1:50-200); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does TMEM67/Meckelin Recombinant Antibody (bsm-62769R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-62769R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

What immunogen was used to raise TMEM67/Meckelin Recombinant Antibody (bsm-62769R)?

KLH conjugated synthetic peptide derived from human Meckelin

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