Phospho-Syk(Tyr323) Recombinant Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62848r-100uL |
| Product Name | Phospho-Syk(Tyr323) Recombinant Antibody |
| Applications |
WB
IHC-P
IHC-F
IF
ICC/IF
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Syk around the phosphorylation site of Y323 |
| Modification Site | Tyr323 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 6850 |
| Swiss Prot | P43405 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | KSYK; p72syk; Spleen tyrosine kinase; Syk. |
| Background | Non-receptor tyrosine kinase which mediates signal transduction downstream of a variety of transmembrane receptors including classical immunoreceptors like the B-cell receptor (BCR). Regulates several biological processes including innate and adaptive immunity, cell adhesion, osteoclast maturation, platelet activation and vascular development.Assembles into signaling complexes with activated receptors at the plasma membrane via interaction between its SH2 domains and the receptor tyrosine-phosphorylated ITAM domains. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF | ICC1:50-200 |
| ICC/IF | IF |
Frequently asked questions
Which applications is Phospho-Syk(Tyr323) Recombinant Antibody (bsm-62848R) validated for, and at what dilution?
bsm-62848R is validated for WB (1:300-5000); IHC-P (1:200-400); IHC-F (1:100-500); IF(ICC) (1:50-200); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-Syk(Tyr323) Recombinant Antibody (bsm-62848R) react with?
Tested reactivity: Human.
How should bsm-62848R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
What immunogen was used to raise Phospho-Syk(Tyr323) Recombinant Antibody (bsm-62848R)?
KLH conjugated synthetic peptide derived from human Syk around the phosphorylation site of Y323