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Phospho-eIF4EBP1/2/3(Thr46/Thr46/Thr32) Recombinant Antibody

Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-eIF4EBP1\/2\/3(Thr46\/Thr46\/Thr32) Monoclonal Antibody, Unconjugated (bsm-62853R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-eIF4EBP1\/2\/3(Thr46\/Thr46\/Thr32) Monoclonal Antibody, Unconjugated (bsm-62853R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-eIF4EBP1\/2\/3(Thr46\/Thr46\/Thr32) Monoclonal Antibody, Unconjugated (bsm-62853R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-eIF4EBP1\/2\/3(Thr46\/Thr46\/Thr32) Monoclonal Antibody, Unconjugated (bsm-62853R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-eIF4EBP1\/2\/3(Thr46\/Thr46\/Thr32) Monoclonal Antibody, Unconjugated (bsm-62853R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-eIF4EBP1\/2\/3(Thr46\/Thr46\/Thr32) Monoclonal Antibody, Unconjugated (bsm-62853R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Pancreatic Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-eIF4EBP1\/2\/3(Thr46\/Thr46\/Thr32) Monoclonal Antibody, Unconjugated (bsm-62853R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Pancreatic Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-eIF4EBP1\/2\/3(Thr46\/Thr46\/Thr32) Monoclonal Antibody, Unconjugated (bsm-62853R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF(IHC-P)

Reactivity

  • Human
  • Mouse
Overview
Catalog # bsm-62853r-100uL
Product Name Phospho-eIF4EBP1/2/3(Thr46/Thr46/Thr32) Recombinant Antibody
Applications
WB IHC-P IHC-F IF(IHC-P)
Reactivity Human, Mouse
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human 4E BP1 around the phosphorylation site of T46
Modification Site Thr46/Thr46/Thr32
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 1978
Swiss Prot Q13541, Q13542, O60516
Subcellular location Cytoplasm, Nucleus
Synonyms 4EBP1; 4EBP2; 4EBP3; eIF4E binding protein 1; eIF4E binding protein 2; eIF4E binding protein 3; PHAS1.
Background eIF4EBP1 is a member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs.
Application Dilution
WB 1:500-2000
IHC-P IHC-P1:50-200
IHC-F IHC-F1:50-200
IF(IHC-P) IFIHC-P1:50-200

Frequently asked questions

Which applications is Phospho-eIF4EBP1/2/3(Thr46/Thr46/Thr32) Recombinant Antibody (bsm-62853R) validated for, and at what dilution?

bsm-62853R is validated for WB(WB) (1:500-2000); IHC-P(IHC-P) (1:50-200); IHC-F(IHC-F) (1:50-200); IF(IHC-P)(IF(IHC-P)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Phospho-eIF4EBP1/2/3(Thr46/Thr46/Thr32) Recombinant Antibody (bsm-62853R) react with?

Tested reactivity: Human, Mouse.

How should bsm-62853R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

What immunogen was used to raise Phospho-eIF4EBP1/2/3(Thr46/Thr46/Thr32) Recombinant Antibody (bsm-62853R)?

KLH conjugated synthetic peptide derived from human 4E BP1 around the phosphorylation site of T46

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