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Myosin heavy chain 2 Recombinant Antibody

Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Myosin heavy chain 2 Monoclonal Antibody, Unconjugated (bsm-62856R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Myosin heavy chain 2 Monoclonal Antibody, Unconjugated (bsm-62856R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Myosin heavy chain 2 Monoclonal Antibody, Unconjugated (bsm-62856R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Myosin heavy chain 2 Monoclonal Antibody, Unconjugated (bsm-62856R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Myosin heavy chain 2 Monoclonal Antibody, Unconjugated (bsm-62856R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Myosin heavy chain 2 Monoclonal Antibody, Unconjugated (bsm-62856R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse tongue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Myosin heavy chain 2 Monoclonal Antibody, Unconjugated (bsm-62856R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse tongue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Myosin heavy chain 2 Monoclonal Antibody, Unconjugated (bsm-62856R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat tongue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Myosin heavy chain 2 Monoclonal Antibody, Unconjugated (bsm-62856R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat tongue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Myosin heavy chain 2 Monoclonal Antibody, Unconjugated (bsm-62856R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with Myosin heavy chain 2 monoclonal antibody, unconjugated (bsm-62856R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with Myosin heavy chain 2 monoclonal antibody, unconjugated (bsm-62856R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-62856r-100uL
Product Name Myosin heavy chain 2 Recombinant Antibody
Applications
WB IHC-P
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Myosin heavy chain 2
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 4620
Swiss Prot Q9UKX2
Subcellular location Cytoplasm
Synonyms IBM3; MYH2; MYH2A; MYHas8; MYHSA2; Myosin heavy chain 2; Myosin heavy chain IIa; MYPOP.
Background Muscle contraction. Required for cytoskeleton organization.
Application Dilution
WB 1:500-2000
IHC-P IHC-P1:100-500

Frequently asked questions

Which applications is Myosin heavy chain 2 Recombinant Antibody (bsm-62856R) validated for, and at what dilution?

bsm-62856R is validated for WB(WB) (1:500-2000); IHC-P(IHC-P) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Myosin heavy chain 2 Recombinant Antibody (bsm-62856R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-62856R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

What immunogen was used to raise Myosin heavy chain 2 Recombinant Antibody (bsm-62856R)?

KLH conjugated synthetic peptide derived from human Myosin heavy chain 2

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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