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Phospho-PKA C alpha/beta/gamma (T197) Recombinant Antibody

Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PKA C alpha\/beta\/gamma (T197) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PKA C alpha\/beta\/gamma (T197) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PKA C alpha\/beta\/gamma (T197) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PKA C alpha\/beta\/gamma (T197) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PKA C alpha\/beta\/gamma (T197) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PKA C alpha\/beta\/gamma (T197) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PKA C alpha\/beta\/gamma (T197) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PKA C alpha\/beta\/gamma (T197) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PKA C alpha\/beta\/gamma (T197) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PKA C alpha\/beta\/gamma (T197) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PKA C alpha\/beta\/gamma (T197) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Phospho-PKA C alpha\/beta\/gamma (T197) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:100 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with Phospho-PKA C alpha\/beta\/gamma (T197) monoclonal antibody, unconjugated (bsm-62860R) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with Phospho-PKA C alpha\/beta\/gamma (T197) monoclonal antibody, unconjugated (bsm-62860R) at 1:500 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T198) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T198) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T198) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T198) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T198) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T198) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T198) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T198) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T198) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T198) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T198) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T198) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T198) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T198) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T198) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PKA C-alpha&beta&gamma (p-T198) Monoclonal Antibody, Unconjugated (bsm-62860R) at 1:200 overnight at 4\u00b0C, followed by incubation with bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF(IHC-P)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-62860r-100uL
Product Name Phospho-PKA C alpha/beta/gamma (T197) Recombinant Antibody
Applications
WB IHC-P IHC-F IF(IHC-P)
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human PKA C alpha around the phosphorylation site of T197
Modification Site Thr197
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 5568
Swiss Prot P22612,P17612,P22694
Subcellular location Cytoplasm, Nucleus
Synonyms cAMP dependent protein kinase catalytic subunit alpha; cAMP dependent protein kinase catalytic subunit beta; cAMP dependent protein kinase catalytic subunit gamma; PKA C alpha; PKA C beta; PKA C gamma; PKACA; PRKACA; PRKACB; PRKACG.
Background Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis.
Application Dilution
WB 1:500-2000
IHC-P IHC-P1:50-200
IHC-F IHC-F1:50-200
IF(IHC-P) IFIHC-P1:50-200

Frequently asked questions

Which applications is Phospho-PKA C alpha/beta/gamma (T197) Recombinant Antibody (bsm-62860R) validated for, and at what dilution?

bsm-62860R is validated for WB(WB) (1:500-2000); IHC-P(IHC-P) (1:50-200); IHC-F(IHC-F) (1:50-200); IF(IHC-P)(IF(IHC-P)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Phospho-PKA C alpha/beta/gamma (T197) Recombinant Antibody (bsm-62860R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-62860R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

What immunogen was used to raise Phospho-PKA C alpha/beta/gamma (T197) Recombinant Antibody (bsm-62860R)?

KLH conjugated synthetic peptide derived from human PKA C alpha around the phosphorylation site of T197

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