Phospho-Nucleolin (Thr76) Recombinant Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-62917r-100uL |
| Product Name | Phospho-Nucleolin (Thr76) Recombinant Antibody |
| Applications |
WB
IHC-P
IHC-F
IF(IHC-P)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NCL around the phosphorylation site of T76 |
| Modification Site | Thr76 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 4691 |
| Swiss Prot | P19338 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | MS1116; NCL; Nucl; Nucleolin; Protein C23. |
| Background | Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. |
| Application Dilution | |
| WB | 1:500-2000 |
| IHC-P | IHC-P1:50-200 |
| IHC-F | IHC-F1:50-200 |
| IF(IHC-P) | IFIHC-P1:50-200 |
Frequently asked questions
Which applications is Phospho-Nucleolin (Thr76) Recombinant Antibody (bsm-62917R) validated for, and at what dilution?
bsm-62917R is validated for WB(WB) (1:500-2000); IHC-P(IHC-P) (1:50-200); IHC-F(IHC-F) (1:50-200); IF(IHC-P)(IF(IHC-P)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-Nucleolin (Thr76) Recombinant Antibody (bsm-62917R) react with?
Tested reactivity: Human.
How should bsm-62917R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
What immunogen was used to raise Phospho-Nucleolin (Thr76) Recombinant Antibody (bsm-62917R)?
KLH conjugated synthetic peptide derived from human NCL around the phosphorylation site of T76