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P53(S33) Recombinant Antibody

4% Paraformaldehyde-fixed HT-29 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (phospho-P53(S33)) monoclonal Antibody, unconjugated (bsm-63095R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed HT-29 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (phospho-P53(S33)) monoclonal Antibody, unconjugated (bsm-63095R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The HT-29 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-phospho-P53(S33) antibody (bsm-63095R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.\r\n
The HT-29 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-phospho-P53(S33) antibody (bsm-63095R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.\r\n
Bioss unconjugated antibody vial

Applications

  • WB
  • ICC/IF

Reactivity

  • Human
Overview
Catalog # bsm-63095r-100uL
Product Name P53(S33) Recombinant Antibody
Applications
WB ICC/IF
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source A synthesized peptide derived from human p53 around the phosphorylation site of S33
Clonality Recombinant
Clone # 7F5
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 7157
Swiss Prot P04637
Subcellular location Cytoplasm, Nucleus
Synonyms TP53 | p53 (phospho-S33); p-p53; phospho-p53; p-TP53; TP53 | p53 (phospho-Ser33); BCC7; BMFS5; LFS1; P53; TRP53; Tp53; bbl; bfy; bhy; p44; P53_BOVIN; Tumor suppressor p53; P53_HUMAN; Antigen NY-CO-13; Phosphoprotein p53; P53_MOUSE; P53_RAT;
Application Dilution
WB =1:1000-2000
ICC/IF ICC/IF=1:50-200

Frequently asked questions

Which applications is P53(S33) Recombinant Antibody (bsm-63095R) validated for, and at what dilution?

bsm-63095R is validated for WB (WB=1:1000-2000); ICC/IF (ICC/IF=1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does P53(S33) Recombinant Antibody (bsm-63095R) react with?

Tested reactivity: Human.

How should bsm-63095R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

What immunogen was used to raise P53(S33) Recombinant Antibody (bsm-63095R)?

A synthesized peptide derived from human p53 around the phosphorylation site of S33

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