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EGFR (Y1092) Recombinant Antibody

4% Paraformaldehyde-fixed A431 (treated with pervanadate (1 mM, 30min)) (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (phospho-EGFR (Y1092)) monoclonal Antibody, unconjugated (bsm-63111R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed A431 (treated with pervanadate (1 mM, 30min)) (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (phospho-EGFR (Y1092)) monoclonal Antibody, unconjugated (bsm-63111R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
Paraformaldehyde-fixed, paraffin embedded Mouse Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-EGFR (Y1092) Monoclonal Antibody, Unconjugated (bsm-63111R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-EGFR (Y1092) Monoclonal Antibody, Unconjugated (bsm-63111R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-EGFR (Y1092) Monoclonal Antibody, Unconjugated (bsm-63111R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-EGFR (Y1092) Monoclonal Antibody, Unconjugated (bsm-63111R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-EGFR (Y1092) Monoclonal Antibody, Unconjugated (bsm-63111R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-EGFR (Y1092) Monoclonal Antibody, Unconjugated (bsm-63111R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-EGFR (Y1092) Monoclonal Antibody, Unconjugated (bsm-63111R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with phospho-EGFR (Y1092) Monoclonal Antibody, Unconjugated (bsm-63111R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
The HeLa (treated with pervanadate (1 mM, 30min)) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-phospho-EGFR (Y1092) antibody (bsm-63111R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The HeLa (treated with pervanadate (1 mM, 30min)) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-phospho-EGFR (Y1092) antibody (bsm-63111R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF
  • ICC/IF

Reactivity

  • Human
  • Mouse
Overview
Catalog # bsm-63111r-100uL
Product Name EGFR (Y1092) Recombinant Antibody
Applications
WB IHC-P IHC-F IF ICC/IF
Reactivity Human, Mouse
Specifications
Conjugation Unconjugated
Host Rabbit
Source A synthesized peptide derived from human EGFR around the phosphorylation site of Y1092
Clonality Recombinant
Clone # 4B5
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 1956
Swiss Prot P00533
Subcellular location Cytoplasm, Nucleus, Secreted, Cell membrane
Synonyms EGFR | ERBB1/HER1 (phospho-Y1092); p-ERBB1/HER1; phospho-ERBB1/HER1; p-EGFR; EGFR | ERBB1/HER1 (phospho-Tyr1092); ERBB; ERBB1; ERRP; HER1; NISBD2; NNCIS; PIG61; mENA; 9030024J15Rik; Errb1; Wa5; wa-2; wa2; ErbB-1; EGFR_HUMAN; EGFR; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; 2.7.10.1; EGFR_MOUSE;
Application Dilution
WB =1:1000-2000
IHC-P IHC-P=1:100-200
IHC-F IHC-F=1:100-200
IF IF=1:50-200
ICC/IF ICC/IF=1:50-200

Frequently asked questions

Which applications is EGFR (Y1092) Recombinant Antibody (bsm-63111R) validated for, and at what dilution?

bsm-63111R is validated for WB (WB=1:1000-2000); IHC-P (IHC-P=1:100-200); IHC-F (IHC-F=1:100-200); IF (IF=1:50-200); ICC/IF (ICC/IF=1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does EGFR (Y1092) Recombinant Antibody (bsm-63111R) react with?

Tested reactivity: Human, Mouse.

How should bsm-63111R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

What immunogen was used to raise EGFR (Y1092) Recombinant Antibody (bsm-63111R)?

A synthesized peptide derived from human EGFR around the phosphorylation site of Y1092

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