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EIF2AK2 Recombinant Antibody

25 ug total protein per lane of various lysates (see on figure) probed with EIF2AK2 monoclonal antibody, unconjugated (bsm-63145R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with EIF2AK2 monoclonal antibody, unconjugated (bsm-63145R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EIF2AK2 Monoclonal Antibody, Unconjugated (bsm-63145R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EIF2AK2 Monoclonal Antibody, Unconjugated (bsm-63145R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EIF2AK2 Monoclonal Antibody, Unconjugated (bsm-63145R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EIF2AK2 Monoclonal Antibody, Unconjugated (bsm-63145R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EIF2AK2 Monoclonal Antibody, Unconjugated (bsm-63145R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EIF2AK2 Monoclonal Antibody, Unconjugated (bsm-63145R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
The A549 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-EIF2AK2 antibody (bsm-63145R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The A549 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-EIF2AK2 antibody (bsm-63145R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IP
  • IF
  • ICC/IF

Reactivity

  • Human
Overview
Catalog # bsm-63145r-100uL
Product Name EIF2AK2 Recombinant Antibody
Applications
WB IHC-P IHC-F IP IF ICC/IF
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source A synthesized peptide derived from human eIF2A K2
Clonality Recombinant
Clone # 3D8
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 5610
Swiss Prot P19525
Subcellular location Cytoplasm, Nucleus
Synonyms DYT33; EIF2AK1; LEUDEN; PKR; PPP1R83; PRKR; E2AK2_HUMAN; EIF2AK2; Eukaryotic translation initiation factor 2-alpha kinase 2 (eIF-2A protein kinase 2); Interferon-inducible RNA-dependent protein kinase; P1/eIF-2A protein kinase; Protein kinase RNA-activated (PKR | Protein kinase R); Tyrosine-protein kinase EIF2AK2; p68 kinase; 2.7.11.1;
Application Dilution
WB =1:500-2000
IHC-P IHC-P=1:100-200
IHC-F IHC-F=1:100-200
IP IP=1:20-50
IF IF=1:50-200
ICC/IF ICC/IF=1:50-200

Frequently asked questions

Which applications is EIF2AK2 Recombinant Antibody (bsm-63145R) validated for, and at what dilution?

bsm-63145R is validated for WB (WB=1:500-2000); IHC-P (IHC-P=1:100-200); IHC-F (IHC-F=1:100-200); IP (IP=1:20-50); IF (IF=1:50-200); ICC/IF (ICC/IF=1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does EIF2AK2 Recombinant Antibody (bsm-63145R) react with?

Tested reactivity: Human.

How should bsm-63145R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

What immunogen was used to raise EIF2AK2 Recombinant Antibody (bsm-63145R)?

A synthesized peptide derived from human eIF2A K2

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