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Di-Methyl-Histone H3 (K9) Monoclonal Antibody

4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Di-Methyl-Histone H3 (K9)) monoclonal Antibody, unconjugated (bsm-63154R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Di-Methyl-Histone H3 (K9)) monoclonal Antibody, unconjugated (bsm-63154R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Di-Methyl-Histone H3 (K9) antibody (bsm-63154R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Di-Methyl-Histone H3 (K9) antibody (bsm-63154R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Bioss unconjugated antibody vial

Applications

  • WB
  • IP
  • ChIP
  • ICC/IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-63154r-100uL
Product Name Di-Methyl-Histone H3 (K9) Monoclonal Antibody
Applications
WB IP CHIP ICC/IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source A synthesized peptide derived from human Histone H3.1 around the methylation site of K80
Clonality Recombinant
Clone # 7D3
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot P68431
Subcellular location Nucleus
Synonyms H3C1 | Histone H3 (me2-K9); me2-Histone H3; di-methyl-Histone H3; H3K9me2; me2-H3;
Application Dilution
WB =1:1000-2000
IP IP=1:20-50
ChIP ChIP=1:20-50
ICC/IF ICC/IF=1:50-200

Frequently asked questions

Which applications is Di-Methyl-Histone H3 (K9) Monoclonal Antibody (bsm-63154R) validated for, and at what dilution?

bsm-63154R is validated for WB (WB=1:1000-2000); IP (IP=1:20-50); ChIP (ChIP=1:20-50); ICC/IF (ICC/IF=1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Di-Methyl-Histone H3 (K9) Monoclonal Antibody (bsm-63154R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-63154R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

What immunogen was used to raise Di-Methyl-Histone H3 (K9) Monoclonal Antibody (bsm-63154R)?

A synthesized peptide derived from human Histone H3.1 around the methylation site of K80

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