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MEK3/MEK6 Monoclonal Antibody

4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (MEK3\/MEK6) monoclonal Antibody, unconjugated (bsm-63167R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (MEK3\/MEK6) monoclonal Antibody, unconjugated (bsm-63167R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-MEK3\/MEK6 antibody (bsm-63167R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-MEK3\/MEK6 antibody (bsm-63167R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
25 ug total protein per lane of various lysates (see on figure) probed with MEK3\/MEK6 monoclonal antibody, unconjugated (bsm-63167R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with MEK3\/MEK6 monoclonal antibody, unconjugated (bsm-63167R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MEK3\/MEK6 Monoclonal Antibody, Unconjugated (bsm-63167R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MEK3\/MEK6 Monoclonal Antibody, Unconjugated (bsm-63167R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MEK3\/MEK6 Monoclonal Antibody, Unconjugated (bsm-63167R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MEK3\/MEK6 Monoclonal Antibody, Unconjugated (bsm-63167R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MEK3\/MEK6 Monoclonal Antibody, Unconjugated (bsm-63167R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MEK3\/MEK6 Monoclonal Antibody, Unconjugated (bsm-63167R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MEK3\/MEK6 Monoclonal Antibody, Unconjugated (bsm-63167R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MEK3\/MEK6 Monoclonal Antibody, Unconjugated (bsm-63167R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MEK3\/MEK6 Monoclonal Antibody, Unconjugated (bsm-63167R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MEK3\/MEK6 Monoclonal Antibody, Unconjugated (bsm-63167R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IP
  • IF
  • Flow-Cyt
  • ICC/IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-63167r-100uL
Product Name MEK3/MEK6 Monoclonal Antibody
Applications
WB IHC-P IHC-F IP IF FLOW-CYT ICC/IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source A synthesized peptide derived from human MEK3
Clonality Recombinant
Clone # 10E1
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot P46734
Subcellular location Cytoplasm, Nucleus
Synonyms MAPKK3; MEK3; MKK3; PRKMK3; SAPKK-2; SAPKK2; MAPKK 3; MKK3b; MP2K3_HUMAN; MAP2K3; MAP kinase kinase 3; MAPK/ERK kinase 3 (MEK 3); Stress-activated protein kinase kinase 2 (SAPK kinase 2 | SAPKK-2 | SAPKK2); 2.7.12.2; SKK2; MP2K3_MOUSE;
Application Dilution
WB =1:1000-2000
IHC-P IHC-P=1:100-200
IHC-F IHC-F=1:100-200
IP IP=1:20-50
IF IF=1:50-200
Flow-Cyt Flow-Cyt=1:50-100
ICC/IF ICC/IF=1:50-200

Frequently asked questions

Which applications is MEK3/MEK6 Monoclonal Antibody (bsm-63167R) validated for, and at what dilution?

bsm-63167R is validated for WB (WB=1:1000-2000); IHC-P (IHC-P=1:100-200); IHC-F (IHC-F=1:100-200); IP (IP=1:20-50); IF (IF=1:50-200); Flow-Cyt (Flow-Cyt=1:50-100); ICC/IF (ICC/IF=1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does MEK3/MEK6 Monoclonal Antibody (bsm-63167R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-63167R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

What immunogen was used to raise MEK3/MEK6 Monoclonal Antibody (bsm-63167R)?

A synthesized peptide derived from human MEK3

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