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FOXO4 Recombinant Antibody

The Jurkat (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-FOXO4 antibody (bsm-63171R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The Jurkat (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-FOXO4 antibody (bsm-63171R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
4% Paraformaldehyde-fixed Jurkat (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (FOXO4) monoclonal Antibody, unconjugated (bsm-63171R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed Jurkat (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (FOXO4) monoclonal Antibody, unconjugated (bsm-63171R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
Bioss unconjugated antibody vial

Applications

  • WB
  • IP
  • Flow-Cyt
  • ICC/IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-63171r-100uL
Product Name FOXO4 Recombinant Antibody
Applications
WB IP FLOW-CYT ICC/IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source A synthesized peptide derived from human FOXO4
Clonality Recombinant
Clone # 4D2
Isotype IgG
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 4303
Swiss Prot P98177
Subcellular location Cytoplasm, Nucleus
Synonyms AFX; AFX1; MLLT7; Afxh; RGD1561201; FOXO4_HUMAN; FOXO4; Fork head domain transcription factor AFX1; FOXO4_MOUSE; Fkhr3; forkhead box O4; myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog); translocated to, 7; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila)
Application Dilution
WB 1:1000-2000
IP 1:20-50
Flow-Cyt 1:50-100
ICC/IF 1:50-200

Frequently asked questions

Which applications is FOXO4 Recombinant Antibody (bsm-63171R) validated for, and at what dilution?

bsm-63171R is validated for WB (1:1000-2000); IP (1:20-50); Flow-Cyt (1:50-100); ICC/IF (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does FOXO4 Recombinant Antibody (bsm-63171R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-63171R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

What immunogen was used to raise FOXO4 Recombinant Antibody (bsm-63171R)?

A synthesized peptide derived from human FOXO4

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