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Mono-Methyl-Histone H3(K9) Monoclonal Antibody

4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Mono-Methyl-Histone H3(K9)) monoclonal Antibody, unconjugated (bsm-63262R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Mono-Methyl-Histone H3(K9)) monoclonal Antibody, unconjugated (bsm-63262R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
25 ug total protein per lane of various lysates (see on figure) probed with Mono-Methyl-Histone H3(K9) monoclonal antibody, unconjugated (bsm-63262R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with Mono-Methyl-Histone H3(K9) monoclonal antibody, unconjugated (bsm-63262R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Mono-Methyl-Histone H3(K9) antibody (bsm-63262R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Cells stained with Primary Antibody for 30 min at room temperature,followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Mono-Methyl-Histone H3(K9) antibody (bsm-63262R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IP
  • IF
  • ChIP
  • ICC/IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-63262r-100uL
Product Name Mono-Methyl-Histone H3(K9) Monoclonal Antibody
Applications
WB IHC-P IHC-F IP IF CHIP ICC/IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source A synthesized peptide derived from human Histone H3.1 around the methylation site of K10
Clonality Recombinant
Clone # 4E13
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot P68431
Subcellular location Nucleus
Synonyms H3C1 | Histone H3 (me1-K10); me-Histone H3; methyl-Histone H3; H3K10me1; me-H3;
Application Dilution
WB =1:500-2000
IHC-P IHC-P=1:100-200
IHC-F IHC-F=1:100-200
IP IP=1:20-50
IF IF=1:50-200
ChIP ChIP=1:20-50
ICC/IF ICC/IF=1:100-500

Frequently asked questions

Which applications is Mono-Methyl-Histone H3(K9) Monoclonal Antibody (bsm-63262R) validated for, and at what dilution?

bsm-63262R is validated for WB (WB=1:500-2000); IHC-P (IHC-P=1:100-200); IHC-F (IHC-F=1:100-200); IP (IP=1:20-50); IF (IF=1:50-200); ChIP (ChIP=1:20-50); ICC/IF (ICC/IF=1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Mono-Methyl-Histone H3(K9) Monoclonal Antibody (bsm-63262R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-63262R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

What immunogen was used to raise Mono-Methyl-Histone H3(K9) Monoclonal Antibody (bsm-63262R)?

A synthesized peptide derived from human Histone H3.1 around the methylation site of K10

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