DiMethyl-Histone H3(K4) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-63264r-100uL |
| Product Name | DiMethyl-Histone H3(K4) Monoclonal Antibody |
| Applications |
WB
IHC-P
IHC-F
IP
IF
CHIP
ICC/IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | A synthesized peptide derived from human Histone H3.1 around the methylation site of K4 |
| Clonality | Recombinant |
| Clone # | 2C11 |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Swiss Prot | P68431 |
| Subcellular location | Nucleus |
| Synonyms | H3C1 | Histone H3 (me2-K5); me2-Histone H3; di-methyl-Histone H3; H3K5me2; me2-H3; |
| Application Dilution | |
| WB | =1:500-2000 |
| IHC-P | IHC-P=1:100-200 |
| IHC-F | IHC-F=1:100-200 |
| IP | IP=1:20-50 |
| IF | IF=1:50-200 |
| ChIP | ChIP=1:20-50 |
| ICC/IF | ICC/IF=1:50-200 |
Frequently asked questions
Which applications is DiMethyl-Histone H3(K4) Monoclonal Antibody (bsm-63264R) validated for, and at what dilution?
bsm-63264R is validated for WB (WB=1:500-2000); IHC-P (IHC-P=1:100-200); IHC-F (IHC-F=1:100-200); IP (IP=1:20-50); IF (IF=1:50-200); ChIP (ChIP=1:20-50); ICC/IF (ICC/IF=1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DiMethyl-Histone H3(K4) Monoclonal Antibody (bsm-63264R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-63264R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
What immunogen was used to raise DiMethyl-Histone H3(K4) Monoclonal Antibody (bsm-63264R)?
A synthesized peptide derived from human Histone H3.1 around the methylation site of K4