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RPS6KA1 Recombinant Antibody

25 ug total protein per lane of various lysates (see on figure) probed with RPS6KA1 monoclonal antibody, unconjugated (bsm-63287R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with RPS6KA1 monoclonal antibody, unconjugated (bsm-63287R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPS6KA1 Monoclonal Antibody, Unconjugated (bsm-63287R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPS6KA1 Monoclonal Antibody, Unconjugated (bsm-63287R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPS6KA1 Monoclonal Antibody, Unconjugated (bsm-63287R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPS6KA1 Monoclonal Antibody, Unconjugated (bsm-63287R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPS6KA1 Monoclonal Antibody, Unconjugated (bsm-63287R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPS6KA1 Monoclonal Antibody, Unconjugated (bsm-63287R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Chronic tonsillitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPS6KA1 Monoclonal Antibody, Unconjugated (bsm-63287R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Chronic tonsillitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPS6KA1 Monoclonal Antibody, Unconjugated (bsm-63287R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IP
  • IF
  • Flow-Cyt
  • ICC/IF

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Pig
Overview
Catalog # bsm-63287r-100uL
Product Name RPS6KA1 Recombinant Antibody
Applications
WB IHC-P IHC-F IP IF FLOW-CYT ICC/IF
Reactivity Human, Mouse, Rat
Predicted Reactivity Pig
Specifications
Conjugation Unconjugated
Host Rabbit
Source A synthesized peptide derived from human p90 S6K alpha 1
Clonality Recombinant
Clone # 5H2
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 6195
Subcellular location Cytoplasm
Synonyms KS6A1_MOUSE; Rps6ka1; S6K-alpha-1; 90 kDa ribosomal protein S6 kinase 1 (p90-RSK 1 | p90RSK1 | p90S6K); MAP kinase-activated protein kinase 1a (MAPK-activated protein kinase 1a | MAPKAP kinase 1a | MAPKAPK-1a); Ribosomal S6 kinase 1 (RSK-1); 2.7.11.1; Mapkapk1a; Rsk1; KS6A1_RAT; HU-1; MAPKAPK1; RSK; p90Rsk;
Application Dilution
WB =1:500-2000
IHC-P IHC-P=1:100-200
IHC-F IHC-F=1:100-200
IP IP=1:20-50
IF IF=1:50-200
Flow-Cyt Flow-Cyt=1:50-100
ICC/IF ICC/IF=1:50-200

Frequently asked questions

Which applications is RPS6KA1 Recombinant Antibody (bsm-63287R) validated for, and at what dilution?

bsm-63287R is validated for WB (WB=1:500-2000); IHC-P (IHC-P=1:100-200); IHC-F (IHC-F=1:100-200); IP (IP=1:20-50); IF (IF=1:50-200); Flow-Cyt (Flow-Cyt=1:50-100); ICC/IF (ICC/IF=1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does RPS6KA1 Recombinant Antibody (bsm-63287R) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Pig.

How should bsm-63287R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

What immunogen was used to raise RPS6KA1 Recombinant Antibody (bsm-63287R)?

A synthesized peptide derived from human p90 S6K alpha 1

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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