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PRKAR2A Monoclonal Antibody

4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (PRKAR2A) monoclonal Antibody, unconjugated (bsm-63288R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (PRKAR2A) monoclonal Antibody, unconjugated (bsm-63288R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
25 ug total protein per lane of various lysates (see on figure) probed with PRKAR2A monoclonal antibody, unconjugated (bsm-63288R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with PRKAR2A monoclonal antibody, unconjugated (bsm-63288R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PRKAR2A Monoclonal Antibody, Unconjugated (bsm-63288R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PRKAR2A Monoclonal Antibody, Unconjugated (bsm-63288R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Adrenal Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PRKAR2A Monoclonal Antibody, Unconjugated (bsm-63288R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Adrenal Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PRKAR2A Monoclonal Antibody, Unconjugated (bsm-63288R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PRKAR2A Monoclonal Antibody, Unconjugated (bsm-63288R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PRKAR2A Monoclonal Antibody, Unconjugated (bsm-63288R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (PRKAR2A) monoclonal Antibody, unconjugated (bsm-63288R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min . DAPI (blue, C02-04002) was used to stain the cell nuclei.
4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (PRKAR2A) monoclonal Antibody, unconjugated (bsm-63288R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min . DAPI (blue, C02-04002) was used to stain the cell nuclei.
4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (PRKAR2A) monoclonal Antibody, unconjugated (bsm-63288R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, then stained with Phalloidin\/BF555 for 30 min at room temperature; DAPI (blue, C02-04002) was used to stain the cell nuclei.
4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (PRKAR2A) monoclonal Antibody, unconjugated (bsm-63288R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, then stained with Phalloidin\/BF555 for 30 min at room temperature; DAPI (blue, C02-04002) was used to stain the cell nuclei.
4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (PRKAR2A) monoclonal Antibody, unconjugated (bsm-63288R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, then stained with Phalloidin\/BF555 for 30 min at room temperature; DAPI (blue, C02-04002) was used to stain the cell nuclei.
4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (PRKAR2A) monoclonal Antibody, unconjugated (bsm-63288R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, then stained with Phalloidin\/BF555 for 30 min at room temperature; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IP
  • IF
  • ICC/IF

Reactivity

  • Human

Predicted Reactivity

  • Cow
Overview
Catalog # bsm-63288r-100uL
Product Name PRKAR2A Monoclonal Antibody
Applications
WB IHC-P IHC-F IP IF ICC/IF
Reactivity Human
Predicted Reactivity Cow
Specifications
Conjugation Unconjugated
Host Rabbit
Source A synthesized peptide derived from human PRKAR2A
Clonality Recombinant
Clone # 2F10
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Swiss Prot P13861
Subcellular location Cytoplasm, Cell membrane
Synonyms PKR2; PRKAR2; 1110061A24Rik; RII(alpha); KAP2_HUMAN; PRKAR2A; KAP2_MOUSE; KAP2_RAT;
Application Dilution
WB =1:1000-2000
IHC-P IHC-P=1:100-200
IHC-F IHC-F=1:100-200
IP IP=1:20-50
IF IF=1:50-200
ICC/IF ICC/IF=1:50-200

Frequently asked questions

Which applications is PRKAR2A Monoclonal Antibody (bsm-63288R) validated for, and at what dilution?

bsm-63288R is validated for WB (WB=1:1000-2000); IHC-P (IHC-P=1:100-200); IHC-F (IHC-F=1:100-200); IP (IP=1:20-50); IF (IF=1:50-200); ICC/IF (ICC/IF=1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does PRKAR2A Monoclonal Antibody (bsm-63288R) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Cow.

How should bsm-63288R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

What immunogen was used to raise PRKAR2A Monoclonal Antibody (bsm-63288R)?

A synthesized peptide derived from human PRKAR2A

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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