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PLK1 Recombinant Antibody

25 ug total protein per lane of various lysates (see on figure) probed with PLK1 monoclonal antibody, unconjugated (bsm-63624R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with PLK1 monoclonal antibody, unconjugated (bsm-63624R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PLK1 Monoclonal Antibody, Unconjugated (bsm-63624R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PLK1 Monoclonal Antibody, Unconjugated (bsm-63624R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PLK1 Monoclonal Antibody, Unconjugated (bsm-63624R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PLK1 Monoclonal Antibody, Unconjugated (bsm-63624R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PLK1 Monoclonal Antibody, Unconjugated (bsm-63624R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PLK1 Monoclonal Antibody, Unconjugated (bsm-63624R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PLK1 Monoclonal Antibody, Unconjugated (bsm-63624R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PLK1 Monoclonal Antibody, Unconjugated (bsm-63624R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PLK1 Monoclonal Antibody, Unconjugated (bsm-63624R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PLK1 Monoclonal Antibody, Unconjugated (bsm-63624R) at 1:200 overnight at 4\u00b0C, followed by conjugation to bs-0295G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • ELISA
  • IHC-F
  • IP
  • IF
  • IHC-P

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
Overview
Catalog # bsm-63624r-100uL
Product Name PLK1 Recombinant Antibody
Applications
WB ELISA IHC-F IP IF IHC-P
Reactivity Human, Mouse
Predicted Reactivity Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Clonality Recombinant
Clone # 9A4
Isotype IgG
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 5347
Swiss Prot P53350
Subcellular location Cytoplasm, Nucleus
Synonyms PLK; STPK13; PLK1_HUMAN; PLK1; Polo-like kinase 1 (PLK-1); Serine/threonine-protein kinase 13 (STPK13); 2.7.11.21; PLK1_MOUSE; PLK1_RAT; polo like kinase 1; polo-like kinase (Drosophila); polo-like kinase 1; Serine/threonine-protein kinase PLK1; PLK-1; Serine/threonine-protein kinase 13
Application Dilution
WB =1:500-2000
ELISA ELISA=1:5000-20000
IHC-F IHC-F=1:200-1000
IP IP=1:50-200
IF IF=1:200-1000
IHC-P IHC-P=1:200-1000

Frequently asked questions

Which applications is PLK1 Recombinant Antibody (bsm-63624R) validated for, and at what dilution?

bsm-63624R is validated for WB (WB=1:500-2000); ELISA (ELISA=1:5000-20000); IHC-F (IHC-F=1:200-1000); IP (IP=1:50-200); IF (IF=1:200-1000); IHC-P (IHC-P=1:200-1000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does PLK1 Recombinant Antibody (bsm-63624R) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat.

How should bsm-63624R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

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