Recombinant SARS Spike RBD

OVERVIEW
CATALOG # bs-46008P
PRODUCT NAME
Recombinant SARS Spike RBD
CROSS REACTIVE SPECIES DETAILS
SARS
CONJUGATION Unconjugated
SOURCE SARS Spike RBD is expressed from mammalian with a His tag and Avi at the C-terminal.
It contains Arg306-Phe527.

EXPRESSION SYSTEM
HEK293
TAG His-Avi
MOLECULAR WEIGHT The protein has a predicted MW of 27.9 kDa. Due to glycosylation, the protein migrates to 36-46kDa based on Bis-Tris PAGE result.
ENDOTOXIN
Less than 1EU per ug by the LAL method.
PURITY: > 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

FORMULATION
Lyophilized from 0.22um filtered solution in 20mM PB (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
STORAGE The product should be stored at -70℃ or -20℃.
TARGET
ACCESSION
P59594
BACKGROUND

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.