Recombinant SARS-CoV-2 Spike RBD

OVERVIEW
CATALOG # bs-46009P
PRODUCT NAME
Recombinant SARS-CoV-2 Spike RBD
CONJUGATION Unconjugated
SOURCE SARS-CoV-2 S protein RBD is expressed from a mammalian cell line with a mouse Fc tag at the C-terminal. 
It contains Arg319-Asn532.

EXPRESSION SYSTEM
HEK293
TAG mFc-Tag
MOLECULAR WEIGHT The protein has a predicted MW of 49.7kDa. Due to glycosylation, the protein migrates to 60-70KDa based on Bis-Tris PAGE result.
ENDOTOXIN
Less than 0.5 EU per ug by the LAL method.
PURITY: > 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC

FORMULATION
Lyophilized from 0.22um filtered solution in 20mM PB (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
STORAGE The product should be stored at -70℃ or -20℃.
TARGET
ACCESSION
QHD43416.1
BACKGROUND The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction. The S protein plays key parts in the induction of neutralizing-antibody and T-cell
responses, as well as protective immunity.