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Recombinant Rabbit Monoclonal Antibodies

Recombinant Rabbit Monoclonal Antibodies


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Catalog #1: bsm-62927r-bf555-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bsm-62927r-bf488-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bsm-62927r-bf405-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bsm-62927r-bf350-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-350

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-PAK1\/2\/3 (Ser144\/Ser141\/Ser154) Monoclonal Antibody, Unconjugated(bsm-62927R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-PAK1\/2\/3 (Ser144\/Ser141\/Ser154) Monoclonal Antibody, Unconjugated(bsm-62927R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-PAK1\/2\/3 (Ser144\/Ser141\/Ser154) Monoclonal Antibody, Unconjugated(bsm-62927R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-PAK1\/2\/3 (Ser144\/Ser141\/Ser154) Monoclonal Antibody, Unconjugated(bsm-62927R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-62927r

Applications: WB, IHC-P, IHC-F, IP, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Phospho-PAK1(S144)+PAK2(S141)+PAK3(S139) Monoclonal Antibody, Unconjugated (bsm-54514R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Phospho-PAK1(S144)+PAK2(S141)+PAK3(S139) Monoclonal Antibody, Unconjugated (bsm-54514R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Phospho-PAK1(S144)+PAK2(S141)+PAK3(S139) Monoclonal Antibody, Unconjugated (bsm-54514R) at 1:50 for 30 minutes at room temperature, DAB staining.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 20 min; Antibody incubation with (Phospho-PAK1(S144)+PAK2(S141)+PAK3(S139)) Monoclonal Antibody, Unconjugated (bsm-54514R) 1:50, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Catalog #1: bsm-54514R

Applications: WB, FCM, IHC-P, IF(ICC), IP, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Hela cells; Lane 2: NIH\/3T3 cells; Lane 3: SH-SY-5Y cells; Probed with Phospho-PAK1 (S144)+PAK2 (S141)+PAK3 (S139) (9A3) Monoclonal Antibody (bsm-52435R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Flow cytometric analysis of nih-3t3 cells with Phospho-PAK1 (S144)+PAK2 (S141)+PAK3 (S139) (9A3) Monoclonal Antibody (bsm-52435R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
IF(ICC) staining with Phospho-PAK1 (S144)+PAK2 (S141)+PAK3 (S139) (9A3) Monoclonal Antibody (bsm-52435R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human liver cancer tissue incubated with Phospho-PAK1 (S144)+PAK2 (S141)+PAK3 (S139) (9A3) Monoclonal Antibody (bsm-52435R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52435R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-52179r-rbitc-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human

Conjugate: RBITC

Catalog #1: bsm-52179r-percp-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bsm-52179r-percp-Cy5.5-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-52179r-percp-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human

Conjugate: PerCP

Catalog #1: bsm-52179r-pe-cy7-100ul

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bsm-52179r-pe-Cy5.5-100ul

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bsm-52179r-pe-cy5-100ul

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: PE-Cy5

Catalog #1: bsm-52179r-pe-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-52179r-fitc-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-52179r-cy7-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-52179r-Cy5.5-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-52179r-cy5-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-52179r-cy3-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-52179r-apc-cy7-100ul

Applications: WB, Flow-Cyt

Reactivity: Human

Conjugate: APC-Cy7

Catalog #1: bsm-52179r-apc-Cy5.5-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bsm-52179r-apc-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human

Conjugate: APC

Catalog #1: bsm-52179r-bf750-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bsm-52179r-bf680-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bsm-52179r-bf647-100ul

Applications: WB, IF

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bsm-52179r-bf594-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bsm-52179r-bf555-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-52179r-bf488-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-52179r-bf405-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-52179r-bf350-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human

Conjugate: AbBy Fluor-350

Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Nrf2(S40) (7G4) Monoclonal Antibody(bsm-52179R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (phospho-Nrf2 (Ser40)) monoclonal Antibody, Unconjugated (bsm-52179R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-Nrf2 (Ser40) Monoclonal Antibody, Unconjugated(bsm-52179R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with phospho-Nrf2 (Ser40) Monoclonal Antibody, Unconjugated(bsm-52179R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.

Catalog #1: bsm-52179r-TR

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-54498R

Applications: WB, FCM, IF(ICC), IP

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Phospho-MEK1(S218\/S222) Monoclonal Antibody, Unconjugated (bsm-54527R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Phospho-MEK1(S218\/S222) Monoclonal Antibody, Unconjugated (bsm-54527R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Phospho-MEK1(S218\/S222) Monoclonal Antibody, Unconjugated (bsm-54527R) at 1:50 for 30 minutes at room temperature, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Phospho-MEK1(S218\/S222) Monoclonal Antibody, Unconjugated (bsm-54527R) at 1:50 for 30 minutes at room temperature, DAB staining.

Catalog #1: bsm-54527R

Applications: WB, FCM, IHC-P, IF(ICC), IP, IHC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-90267r-50µL

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-52462r-rbitc-100ul

Applications: IF

Reactivity: Human

Conjugate: RBITC

Catalog #1: bsm-52462r-percp-Cy5.5-100ul

Applications: IF

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-52462r-percp-100ul

Applications: IF

Reactivity: Human

Conjugate: PerCP

Catalog #1: bsm-52462R-PE-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bsm-52462R-PE-Cy5.5

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bsm-52462R-PE-Cy5

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5

Catalog #1: bsm-52462r-pe-100ul

Applications: IF

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-52462r-fitc-100ul

Applications: IF

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-52462r-cy7-100ul

Applications: IF

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-52462r-Cy5.5-100ul

Applications: IF

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-52462r-cy5-100ul

Applications: IF

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-52462r-cy3-100ul

Applications: IF

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-52462R-APC-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7