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Recombinant Rabbit Monoclonal Antibodies



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Catalog #1: bsm-61103r-pe-cy7

Applications: WB, FCM

Reactivity: Human, Mouse

Conjugate: PE-Cy7

Catalog #1: bsm-61103r-bf750

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-750

Catalog #1: bsm-61103r-Cy5.5

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy5.5

Catalog #1: bsm-61103r-apc-Cy5.5

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse

Conjugate: APC-Cy5.5

Catalog #1: bsm-61103r-bf680

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-680

Catalog #1: bsm-61103r-cy7

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy7

Catalog #1: bsm-61103r-bf488

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-488

Catalog #1: bsm-61103r-bf647

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-647

Catalog #1: bsm-61103r-bf350

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-350

Catalog #1: bsm-61103r-bf555

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-555

Catalog #1: bsm-61103r-apc

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse

Conjugate: APC

Catalog #1: bsm-61103r-bf405

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-405

Catalog #1: bsm-61103r-apc-cy7

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse

Conjugate: APC-Cy7

Catalog #1: bsm-61103r-bf594

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-594

Catalog #1: bsm-61100r-percp-Cy5.5

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-61100r-pe-cy5

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: PE-Cy5

Catalog #1: bsm-61100r-cy7

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: Cy7

Catalog #1: bsm-61100r-pe-cy7

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: PE-Cy7

Catalog #1: bsm-61100r-Cy5.5

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: Cy5.5

Catalog #1: bsm-61100r-pe-Cy5.5

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: PE-Cy5.5

Catalog #1: bsm-61100r-bf594

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-594

Catalog #1: bsm-61100r-bf350

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-350

Catalog #1: bsm-61100r-bf488

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-488

Catalog #1: bsm-61100r-percp

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: PerCP

Catalog #1: bsm-61100r-cy3

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: Cy3

Catalog #1: bsm-61100r-bf680

Applications: WB

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-680

Western blot analysis of (1) 293 cell lysate; (2) HeLa cell lysate. Using RNF20 (bsm-61100R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n
The HepG2 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-RNF20 antibody (bsm-61100R,1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (bs-60295G-BF488): 1 \u03bcg\/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-61100r

Applications: WB, IP, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-61100r-bf555

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-555

Catalog #1: bsm-61100r-apc

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: APC

Catalog #1: bsm-61100r-bf405

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-405

Catalog #1: bsm-61100r-cy5

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: Cy5

Catalog #1: bsm-61100r-bf750

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-750

Catalog #1: bsm-61100r-fitc

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: FITC

Catalog #1: bsm-61100r-bf647

Applications: WB

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-647

Catalog #1: bsm-61100r-rbitc

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: RBITC

Catalog #1: bsm-61100r-apc-cy7

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: APC-Cy7

Catalog #1: bsm-61100r-pe

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: PE

Catalog #1: bsm-61100r-apc-Cy5.5

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: APC-Cy5.5

25 ug total protein per lane of various lysates (see on figure) probed with UCHL3 monoclonal antibody, unconjugated (bsm-63011R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The U-87MG (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-UCHL3 antibody (bsm-63011R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with UCHL3 Monoclonal Antibody, Unconjugated (bsm-63011R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with UCHL3 Monoclonal Antibody, Unconjugated (bsm-63011R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-63011r

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-63022r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-63002r

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62988r

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-62922r

Applications: WB, FCM, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

4% Paraformaldehyde-fixed Raji (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (CD10) monoclonal Antibody, unconjugated (bsm-62909R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The Raji (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.) , followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-CD10 antibody (bsm-62909R): 1 \u03bcg\/10^6 cells; Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
25 ug total protein per lane of various lysates (see on figure) probed with CD10 monoclonal antibody, unconjugated (bsm-62909R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-62909r

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62914r

Applications: WB, IP, Flow-Cyt

Reactivity: Human

Conjugate: Unconjugated

4% Paraformaldehyde-fixed Raji (H) cell; Antibody incubation with (ITCH\/AIP4) monoclonal Antibody, unconjugated (bsm-62844R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The Raji (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.) , followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-ITCH\/AIP4 antibody (bs-62844R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-62844r

Applications: FCM, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with SHIP1 monoclonal antibody, unconjugated (bsm-62832R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SHIP1 Monoclonal Antibody, Unconjugated (bsm-62832R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with SHIP1 Monoclonal Antibody, Unconjugated (bsm-62832R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-62832r

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IP

Reactivity: Human

Conjugate: Unconjugated

The Jurkat (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-NR2C2 antibody (bsm-62803R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
25 ug total protein per lane of various lysates (see on figure) probed with NR2C2 monoclonal antibody, unconjugated (bsm-62803R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-62803r

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated