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Recombinant Rabbit Monoclonal Antibodies

Recombinant Rabbit Monoclonal Antibodies


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Catalog #1: bsm-62100r

Applications: WB, IHC-P, IHC-F, IP, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-61505r-biotin-100ul

Applications: WB, IHC-P, IHC-F, IP

Reactivity: Mouse, Rat

Conjugate: Biotin

25 ug total protein per lane of various lysates (see on figure) probed with alpha Sarcoglycan monoclonal antibody, unconjugated (bsm-61505R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with alpha Sarcoglycan Monoclonal Antibody, Unconjugated (bsm-61505R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with alpha Sarcoglycan Monoclonal Antibody, Unconjugated (bsm-61505R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with alpha Sarcoglycan Monoclonal Antibody, Unconjugated (bsm-61505R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-61505r

Applications: WB, IHC-P, IHC-F, IP, IF

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62120r-biotin-100ul

Applications: WB, IP

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-62120r

Applications: WB, IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-61736r-biotin-100ul

Applications: WB, IHC-P, IHC-F, IP

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-61736r

Applications: WB, IHC-P, IHC-F, IP, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-61511r-biotin-100ul

Applications: WB, IHC-P, IHC-F, IP

Reactivity: Mouse, Rat

Conjugate: Biotin

25 ug total protein per lane of various lysates (see on figure) probed with Alpha B Crystallin monoclonal antibody, unconjugated (bsm-61511R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Alpha B Crystallin Monoclonal Antibody, Unconjugated (bsm-61511R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Alpha B Crystallin Monoclonal Antibody, Unconjugated (bsm-61511R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Huma Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Alpha B Crystallin Monoclonal Antibody, Unconjugated (bsm-61511R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-61511r

Applications: WB, IHC-P, IHC-F, IP, IF, ICC/IF

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-61162r-biotin-100ul

Applications: WB, IHC-P, IHC-F, IP

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-61162r

Applications: WB, IHC-P, IHC-F, IP, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-61151r-biotin-100ul

Applications: WB, IP

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-61151r-TR

Applications: WB, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-61150r-biotin-100ul

Applications: WB, IP

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-61149r-biotin-100ul

Applications: WB, IHC-P, IHC-F, IP

Reactivity: Human, Mouse

Conjugate: Biotin

The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-AKT1S1 antibody (bsm-61149R,1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (bs-60295G-BF488): 1 \u03bcg\/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (AKT1S1) monoclonal Antibody, unconjugated (bsm-61149R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Catalog #1: bsm-61149r

Applications: WB, IHC-P, IHC-F, IP, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with AKT1S1 monoclonal antibody, unconjugated (bsm-61150R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with AKT1S1 monoclonal antibody, unconjugated (bsm-61150R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-61150r-TR

Applications: WB, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62035r-biotin-100ul

Applications: WB, IP

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-62035r

Applications: WB, IP, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-61280r-biotin-100ul

Applications: WB, IP

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-61280r-TR

Applications: WB, IP, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62145r-biotin-100ul

Applications: WB, IP

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-62145r

Applications: WB, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-61290r-biotin-100ul

Applications: WB, IP

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-61290r-TR

Applications: WB, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62667r-biotin-100ul

Applications: WB, IHC-P, IHC-F, IP

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-62667r

Applications: WB, IHC-P, IHC-F, IP, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62215r-biotin-100ul

Applications: WB, IP

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bsm-62215r

Applications: WB, IP, ICC/IF

Reactivity: Human, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ADAM10 Monoclonal Antibody, Unconjugated(bsm-52935R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with ADAM10 monoclonal antibody, unconjugated (bsm-52935R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-52935r

Applications: WB, IHC-P, IHC-F, IP, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-62757r-biotin-100ul

Applications: WB, IHC-P, IHC-F, IP

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-62757r

Applications: WB, IHC-P, IHC-F, IP, IF, Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Mouse Heart; Probed with ACTN2 (7G3) Monoclonal Antibody (bsm-54465R) at 1:500, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Rat Heart tissue incubated with ACTN2 (7G3) Monoclonal Antibody (bsm-54465R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.
Paraformaldehyde-fixed and paraffin-embedded Human Colon tissue incubated with ACTN2 (7G3) Monoclonal Antibody (bsm-54465R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.
Paraformaldehyde-fixed and paraffin-embedded Human Fetal Skeletal Muscle tissue incubated with ACTN2 (7G3) Monoclonal Antibody (bsm-54465R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.

Catalog #1: bsm-54465R

Applications: WB, IHC-P, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62407r-biotin-100ul

Applications: WB, IP

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-62407r

Applications: WB, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-52832r

Applications: WB, IP

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: Unconjugated

Catalog #1: bsm-62432r-biotin-100ul

Applications: WB, IHC-P, IHC-F, IP

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

25 ug total protein per lane of various lysates (see on figure) probed with ACSL4 monoclonal antibody, unconjugated (bsm-62432R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation ACSL4 Monoclonal Antibody, Unconjugated(bsm-62432R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation ACSL4 Monoclonal Antibody, Unconjugated(bsm-62432R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation ACSL4 Monoclonal Antibody, Unconjugated(bsm-62432R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-62432r

Applications: WB, IHC-P, IHC-F, IP, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-61944r-biotin-100ul

Applications: WB, IHC-P, IHC-F, IP

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-61944r

Applications: WB, IHC-P, IHC-F, IP, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: SiHa Cells; Lane 2: HL-60 Cells; Probed with Aconitase 2 (2F4) Monoclonal Antibody (bsm-54442R) at 1:2000, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Aconitase 2 (2F4) Monoclonal Antibody (bsm-54442R) at 1:100 in SiHa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Rat Epididymis tissue incubated with Aconitase 2 (2F4) Monoclonal Antibody (bsm-54442R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Prostate Cancer tissue incubated with Aconitase 2 (2F4) Monoclonal Antibody (bsm-54442R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54442R

Applications: WB, IHC-P, IF(IHC-P), IF(ICC), IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62460r-biotin-100ul

Applications: WB, IHC-P, IHC-F, IP

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

4% Paraformaldehyde-fixed SH-SY5Y(H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Acetyl-Histone H3.1 (Lys28)) monoclonal Antibody, unconjugated (bsm-62460R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
25 ug total protein per lane of various lysates (see on figure) probed with Acetyl-Histone H3.1 (Lys28) monoclonal antibody, unconjugated (bsm-62460R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The HepG2 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-Acetyl-Histone H3.1 (Lys28) antibody (bsm-62460R): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-60295G-FITC): 1 \u03bcg\/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Acetyl-Histone H3.1 (Lys28) Monoclonal Antibody, Unconjugated(bsm-62460R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.

Catalog #1: bsm-62460r

Applications: WB, IHC-P, IHC-F, IP, IF, ChIP, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-62436r-biotin-100ul

Applications: WB, IP

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bsm-62436r

Applications: WB, IP, ChIP, ICC/IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-62426r-biotin-100ul

Applications: WB, IHC-P, IHC-F, IP

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bsm-62426r

Applications: WB, IHC-P, IHC-F, IP, IF, ChIP, ICC/IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-62678r-biotin-100ul

Applications: WB, IHC-P, IHC-F, IP

Reactivity: Human, Mouse, Rat

Conjugate: Biotin