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Recombinant Rabbit Monoclonal Antibodies

Recombinant Rabbit Monoclonal Antibodies


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Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: HeLa, N2a, BRL, Mouse liver, Mouse kidney\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30\r\nPredicted MW: 23Da\r\nObserved MW: 23Da\r\n
Tissue: Rat liver\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60167R
Tissue: mouse liver\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60167R
Tissue: Human lung squamous carcinoma\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60167R

Catalog #1: bsm-60167r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-90492r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse liver lysate probed with Ubiquitin-like modifier-activating enzyme 1 (8A4) Monoclonal Antibody (bsm-52695R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Ubiquitin-like modifier-activating enzyme 1 (8A4) Monoclonal Antibody (bsm-52695R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Ubiquitin-like modifier-activating enzyme 1 (8A4) Monoclonal Antibody (bsm-52695R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with Ubiquitin-like modifier-activating enzyme 1 (8A4) Monoclonal Antibody (bsm-52695R) at 1:300, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52695R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90030r-50µL

Applications: WB, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90506r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90488r-50µL-ab-25ug-cell-lysate-25ug-wt-cell

Applications: WB, FCM, IC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-90184r-50µL

Applications: WB, FCM, IC

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-63008r-pe-cy3

Applications: WB, FCM

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy3

Catalog #1: bsm-63008r-apc-cy5

Applications: WB, FCM, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5

Catalog #1: bsm-63008r-rbitc-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: RBITC

Catalog #1: bsm-63008r-percp-cy7-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy7

Catalog #1: bsm-63008r-percp-Cy5.5-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-63008r-percp-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bsm-63008r-pe-cy7-100ul

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy7

Catalog #1: bsm-63008r-pe-Cy5.5-100ul

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5.5

Catalog #1: bsm-63008r-pe-cy5-100ul

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: PE-Cy5

Catalog #1: bsm-63008r-pe-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: PE

Catalog #1: bsm-63008r-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-63008r-fitc-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-63008r-cy7-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-63008r-Cy5.5-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-63008r-cy5-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-63008r-cy3-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-63008r-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-63008r-apc-cy7-100ul

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy7

Catalog #1: bsm-63008r-apc-Cy5.5-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: APC-Cy5.5

Catalog #1: bsm-63008r-apc-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: APC

Catalog #1: bsm-63008r-bf750-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-750

Catalog #1: bsm-63008r-bf680-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-680

Catalog #1: bsm-63008r-bf647-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-647

Catalog #1: bsm-63008r-bf594-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-594

Catalog #1: bsm-63008r-bf555-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-555

Catalog #1: bsm-63008r-bf488-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-488

Catalog #1: bsm-63008r-bf405-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-405

Catalog #1: bsm-63008r-bf350-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: AbBy Fluor-350

Western blot analysis of HepG2 cell lysate. Using Ubiquitin (bsm-63008R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ubiquitin Monoclonal Antibody, Unconjugated(bsm-63008R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ubiquitin Monoclonal Antibody, Unconjugated(bsm-63008R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ubiquitin Monoclonal Antibody, Unconjugated(bsm-63008R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining and DAB (C-0010) staining.

Catalog #1: bsm-63008r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-61330r-pe-cy3

Applications: WB

Reactivity: Human, Mouse

Conjugate: PE-Cy3

Catalog #1: bsm-61330r-apc-cy5

Applications: WB, IF(ICC)

Reactivity: Human, Mouse

Conjugate: APC-Cy5

Catalog #1: bsm-61330r-rbitc-100ul

Applications: WB, IF

Reactivity: Human, Mouse

Conjugate: RBITC

Catalog #1: bsm-61330r-percp-cy7-100ul

Applications: WB

Reactivity: Human, Mouse

Conjugate: PerCP-Cy7

Catalog #1: bsm-61330r-percp-Cy5.5-100ul

Applications: WB, IF

Reactivity: Human, Mouse

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-61330r-percp-100ul

Applications: WB, IF

Reactivity: Human, Mouse

Conjugate: PerCP

Catalog #1: bsm-61330r-pe-cy7-100ul

Applications: WB

Reactivity: Human, Mouse

Conjugate: PE-Cy7

Catalog #1: bsm-61330r-pe-Cy5.5-100ul

Applications: WB

Reactivity: Human, Mouse

Conjugate: PE-Cy5.5

Catalog #1: bsm-61330r-pe-cy5-100ul

Applications: WB

Reactivity: Human, Mouse

Conjugate: PE-Cy5

Catalog #1: bsm-61330r-pe-100ul

Applications: WB, IF

Reactivity: Human, Mouse

Conjugate: PE

Catalog #1: bsm-61330r-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bsm-61330r-fitc-100ul

Applications: WB, IF

Reactivity: Human, Mouse

Conjugate: FITC